Research article
A New extractive spectrophotometric method for determination of rizatriptan dosage forms using bromocresol green
1 Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
2 Department of Pharmaceutics, Food and Drug Laboratory Research Center, Ministry of Health, Tehran, Iran
DARU Journal of Pharmaceutical Sciences 2013, 21:12 doi:10.1186/2008-2231-21-12
Published: 2 February 2013Abstract
Background and the purpose of the study
Rizatriptan is used effectively for the treatment of migraine headache. In this study, a simple, rapid and low cost spectrophotometric method based on the ion-pair complexation is proposed for the determination of rizatriptan in raw material and dosage forms.
Methods
The ion-pair complexation using bromocresol green as reagent was performed in a buffer solution and the absorbance was measured by a spectrophotometer. The ion-pair formation conditions were optimized and the accuracy and precision of the method were calculated.
Results and major conclusion
Best results were achieved by using 6 ml of the bromocresol green reagent in the presence of phosphate buffer (pH 3.0). The stoichiometry of the resulted complex was 1:1. The within-day and between-day precision values were lower than 2.9 and 1.8 percent for the calibration range of 0.5–50 and 10–100 μg/ml, respectively. The proposed method was successfully used for the determination of rizatriptan in dosage forms without any interference.
